VANGUARD Plus 5/CV is a freeze-dried preparation of attenuated strains of CD virus, CAV-2, CPI virus, and CPV, plus a liquid preparation of inactivated CCV with an adjuvant. The CPV fraction is high titer (>107.0 TCID50/dose) and was attenuated by low passage (35 passes from the canine isolate with a maximum of 2 additional passes allowed for production) on the canine cell line which gives it the immunogenic properties capable of overriding maternal antibody interference at the levels indicated in Table 2. Some puppies in the field may have higher levels of maternal antibodies than those evaluated in our pivotal efficacy study. All viruses were propagated on established cell lines. The liquid component is used to rehydrate the freeze-dried component, which is packaged with inert gas in place of vacuum.